WuXi AppTec offers the highly accurate and robust characterization of protein stability by analyzing thermal unfolding, aggregation and chemical denaturation using nanoDSF technology – an advanced Differential Scanning Fluorometry (DSF) method.
Advantages of nanoDSF:
WuXi AppTec offers a broad application range for antibodies and membrane proteins:
The development of biologics requires precise biophysical analytics at all stages in the process. With nanoDSF we measure protein melting curves that are truly label free by analyzing intrinsic tryptophan fluorescence. By using nanoDSF, proteins can be measured in any kind of buffer containing any type of additives (e.g. detergents). Moreover, the dynamic range of simultaneously measurable concentrations spans from 150 mg/ml down to 5 μg/ml in a working volume of 10 μl. The technology is used for analysis of protein quality and stability, with applications including protein engineering, formulation development and batch to batch comparison.
For our clients we can analyze:
WuXi AppTec is currently operating the Prometheus NT.48 from NanoTemper with its advantageous low sample consumption and a broad concentration range from ng/mL to mg/ml.